Combine 20 mL of alcohol to the sodium hydroxide/bromothymol blue solution. Dilute the mixture with 1 L of distilled water in a large container. The solution should be dark blue in color. If the solution appears green, use a pipette to slowly add sodium hydroxide drop by drop to the solution until it changes to blue.
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How do you make bromophenol blue solution?

Dissolve 5.0 g of bromophenol blue powder (tetrabromophenolsulfonphthalein) in 74.5 mL of 0.1 N sodium hydroxide (NaOH) solution. Dilute with purified water to 500 mL. Color and pH range: yellow 3.0-4.6 blue.

How do you dilute a BTB?

We recommend diluting the BTB in a 10:1 solution with water as it will conserve your stock. BTB will work the same way when it is more diluted, but the color will appear lighter.

How do you make a 0.04 bromothymol blue solution?

Dissolve bromthymol blue in NaOH. Dilute to 500 ml with distilled water.

How does BTB react with water?

BTB is an acid indicator; when it reacts with acid it turns from blue to yellow. When carbon dioxide reacts with water, a weak acid (carbonic acid) is formed (see chemical reaction below). The more carbon dioxide you breathe into the BTB solution, the faster it will change color to yellow.

How do you make methylene blue solution from powder?

Methylene blue: Prepare a saturated solution of methylene blue by adding 1.5 g powdered methy- lene blue to 100 mL 95% ethyl alcohol. Slowly add the alcohol to dissolve the powder. Add 30 mL saturated alcoholic solution of methylene blue to 100.0 mL distilled water and 0.1 mL 10% potassium hydroxide.

What is bromophenol blue solution?

Bromophenol Blue is a tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

How do you make 0.02 M sodium hydroxide?

If you add 0.64 g of solid sodium hydroxide to the stock solution you’ll get a 0.2-M solution of (approximately) the same volume.

How do you make a mo indicator?

Indicator17 Methyl yellow
pH Range2.9-4.0

How do you make a pop indicator?

Dissolve 10.0 g of phenolphthalein powder in 750 mL of ethanol and dilute to 1 L with distilled water. Add 0.1 N sodium hydroxide (NaOH) solution dropwise to the first permanent faint pink end point. Color and pH range: colorless 8.3-10.0 red.

What is BTB chemistry?

Bromothymol blue (also known as bromothymol sulfone phthalein and BTB) is a pH indicator. It is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). … It is typically sold in solid form as the sodium salt of the acid indicator.

What does a color change in BTB indicate about a solution?

BTB is an acid indicator; when it reacts with acid it turns from blue to yellow. When carbon dioxide reacts with water, a weak acid (carbonic acid) is formed (see chemical reaction below). The more carbon dioxide you breathe into the BTB solution, the faster it will change color to yellow.

When the bromothymol blue BTB was exposed to the co2 gas What color was the solution did the BTB indicate presence of an acid or base?

Bromothymol blue (BMB) is an indicator dye that turns yellow in the presence of acid. When carbon dioxide is added to the solution, it creates carbonic acid, lowering the pH of the solution. BMB is blue when the pH is greater than 7.6, green when the pH is between 6-7.6, and yellow when the pH is less than 6.

How do you make methylene blue reagent?

Dissolve 0.3 g of Methylene blue dye with 30 mL of 95% methanol. Mix with 100 mL of 1% aqueous potassium hidroxyde. The mentioned formulation is only one of the ways of preparing the dye solution. Methylene Blue is most commonly used as a part of May- Gruenwald and Giemsa dyes.

What are the stains used in hematology?

Romanowsky stain solutions are used in hematology. They are composed of methylene blue, oxidative products of methylene blue (Azure A, Azure B, Azure C, and Thionin) and eosin dyes. Giemsa, a commonly used stain, does not adequately stain red blood cells, platelets, or white blood cell cytoplasms when used alone.

What stain is used for reticulocyte?

New methylene blue stains the reticulofilamentous material in reticulocytes more deeply and more uniformly than does brilliant cresyl blue, which varies from sample to sample in its staining ability.

How do you make 6X bromophenol blue?

PROCEDURE. To prepare 10 ml of 6X DNA loading dye, weigh out 25 mg bromophenol blue. Transfer it to a 15-mL screw-capped graduated tube. Add 7.06 ml of 85% Glycerol and 2.94 ml deionized / Milli-Q water.

What color is BPB?

Bromophenol Blue (BPB) test paper is useful in differentiating between a solution with an acidic pH and one with a more neutral pH. At pH 3.0 and lower, the paper will be a yellow/green color. Above pH 4.6, the paper will remain blue (it is blue when unreacted).

What is a loading dye?

Loading dye is mixed with samples for use in gel electrophoresis. It generally contains a dye to assess how “fast” your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).

How do you make a 0.02 M solution?

  1. Dissolve 3.2 g of potassium permanganate in 1000 ml of water.
  2. Heat on a water-bath for 1 hour.
  3. Allow to stand for 2 days and filter through glass wool.
  4. Standardize the solution in the following manner.
How do you make 2 normal NaOH?

For 2N NaOH we have to dissolve 4×2=8grm of NaOH to 100 ml water. Thus by dissolving 8grm of NaOH in 100ml of water we will get 2N NaOH solution.

How do you make 1N sodium hydroxide?

Dissolve 4.5 g of sodium hydroxide in 100 mL distilled water, allow to cool, and then add saturated barium hydroxide solution drop wise with stirring until a precipitate is formed.

How do you make a phenol red indicator solution?

Phenol Red Indicator Solution: Dissolve 0.1 g of phenol red in 2.82 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). After the solution is effected, add sufficient water to produce 100 ml.

How does methyl red work as an indicator?

Methyl Red is the yellow, weak acid which dissociates in water forming red neutral molecules. … Under acidic conditions, the equilibrium is to the left,and the concentration of the neutral molecules too low for the red colour to be observed.

Why is thymol blue used as an indicator?

Thymol blue is another weak acid that is used as an indicator. … The ionization reactions for thymol blue are shown below: At any pH, the color of a thymol blue solution can be predicted using the acid ionization constants. For example, at a pH of 4, the [H+] is 1 x 10-4.

How can I make phenolphthalein at home?

  1. – Weigh out 0.5 g of phenolphthalein.
  2. – Prepare a 50% ethanol (ethyl alcohol) solution consisting of 50ml ethanol and 50 ml water.
  3. – Dissolve the phenolphthalein thoroughly in the 50% ethanol solution.
  4. – Use from a bottle fitted with an eye dropper.
Which solution would phenolphthalein turn pink?

Phenolphthalein is naturally colorless but turns pink in alkaline solutions. The compound remains colorless throughout the range of acidic pH levels but begins to turn pink at a pH level of 8.2 and continues to a bright magenta at pH 10 and above.

Can methanol be used to prepare phenolphthalein?

Phenolphthalein solution 0.375% in methanol.

Is thymol blue the same as Bromothymol blue?

IndicatorThymol blue (second transition)Low pH coloryellowTransition pH range8.0–9.6High pH colorblue

Why is Bromothymol blue green in neutral solutions?

Bromothymol blue acts as a weak acid in solution. … It is bluish green in neutral solution. The deprotonation of the neutral form results in a highly conjugated structure, accounting for the difference in color. An intermediate of the deprotonation mechanism is responsible for the greenish color in neutral solution.

How long does it take for BTB to change color?

Pour the diluted bromothymol blue solution into a beaker. Place a straw in the liquid and exhale gently into it. Allow the exhaled air to bubble through the liquid until the solution turns yellow-green. This will take 15 to 30 minutes.

What gas or gases can BTB serve as an indicator for?

2. Explain that BTB is an indicator that can be used to test for the presence and relative concentration of carbon dioxide.

Why did the color of the bromothymol blue solution change in certain test tubes?

Why did the color of the Bromthymol Blue (BTB) solution change in certain test tubes? The levels of carbon dioxide changed, therefore the BTB solution changed color to indicate the presence of carbon dioxide.

Why do you cover one test tube in aluminum foil?

Why cover one test tube in aluminum foil? The foil blocks light.

What happens when CO2 is added to lime water?

Reaction with limewater Carbon dioxide reacts with limewater (a solution of calcium hydroxide, Ca(OH) 2), to form a white precipitate (appears milky) of calcium carbonate, CaCO 3. Adding more carbon dioxide results in the precipitate dissolving to form a colourless solution of calcium hydrogencarbonate.

Why did the BTB solution in jar C stay blue?

The Elodea will take up the CO2 and use it to produce sugars during photosynthesis, releasing oxygen as a byproduct. Because of the balanced production and uptake of CO2, the BTB will remain blue.

Can methylene blue be taken orally?

Methylene Blue (methylene blue) injection is a form of hemoglobin, a substance in blood, used to treat methemoglobinemia. Methylene Blue oral is used to treat methemoglobinemia and urinary tract infections.

How do you stain a slide with methylene blue?

  1. Take a clean cotton swab and gently scrape the inside of your mouth.
  2. Smear the cotton swab on the centre of the microscope slide for 2 to 3 seconds.
  3. Add a drop of methylene blue solution and place a coverslip on top. …
  4. Remove any excess solution by allowing a paper towel to touch one side of the coverslip.
How do you make basic Fuchsin stain?

Dissolve 0.5 g basic fuchsin dye in 20 ml 95% ethanol. Dilute to 100 ml with distilled water. Filter if necessary with Whatman No. 31 filter paper to remove any undissolved dye.