How do you make a BTB solution?

Dissolve 5.0 g of bromophenol blue powder (tetrabromophenolsulfonphthalein) in 74.5 mL of 0.1 N sodium hydroxide (NaOH) solution. Dilute with purified water to 500 mL. Color and pH range: yellow 3.0-4.6 blue.

We recommend diluting the BTB in a 10:1 solution with water as it will conserve your stock. BTB will work the same way when it is more diluted, but the color will appear lighter.

Dissolve bromthymol blue in NaOH. Dilute to 500 ml with distilled water.

BTB is an acid indicator; when it reacts with acid it turns from blue to yellow. When carbon dioxide reacts with water, a weak acid (carbonic acid) is formed (see chemical reaction below). The more carbon dioxide you breathe into the BTB solution, the faster it will change color to yellow.

Methylene blue: Prepare a saturated solution of methylene blue by adding 1.5 g powdered methy- lene blue to 100 mL 95% ethyl alcohol. Slowly add the alcohol to dissolve the powder. Add 30 mL saturated alcoholic solution of methylene blue to 100.0 mL distilled water and 0.1 mL 10% potassium hydroxide.

Bromophenol Blue is a tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

If you add 0.64 g of solid sodium hydroxide to the stock solution you’ll get a 0.2-M solution of (approximately) the same volume.

Dissolve 10.0 g of phenolphthalein powder in 750 mL of ethanol and dilute to 1 L with distilled water. Add 0.1 N sodium hydroxide (NaOH) solution dropwise to the first permanent faint pink end point. Color and pH range: colorless 8.3-10.0 red.

Bromothymol blue (also known as bromothymol sulfone phthalein and BTB) is a pH indicator. It is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). … It is typically sold in solid form as the sodium salt of the acid indicator.

BTB is an acid indicator; when it reacts with acid it turns from blue to yellow. When carbon dioxide reacts with water, a weak acid (carbonic acid) is formed (see chemical reaction below). The more carbon dioxide you breathe into the BTB solution, the faster it will change color to yellow.

Bromothymol blue (BMB) is an indicator dye that turns yellow in the presence of acid. When carbon dioxide is added to the solution, it creates carbonic acid, lowering the pH of the solution. BMB is blue when the pH is greater than 7.6, green when the pH is between 6-7.6, and yellow when the pH is less than 6.

Dissolve 0.3 g of Methylene blue dye with 30 mL of 95% methanol. Mix with 100 mL of 1% aqueous potassium hidroxyde. The mentioned formulation is only one of the ways of preparing the dye solution. Methylene Blue is most commonly used as a part of May- Gruenwald and Giemsa dyes.

Romanowsky stain solutions are used in hematology. They are composed of methylene blue, oxidative products of methylene blue (Azure A, Azure B, Azure C, and Thionin) and eosin dyes. Giemsa, a commonly used stain, does not adequately stain red blood cells, platelets, or white blood cell cytoplasms when used alone.

New methylene blue stains the reticulofilamentous material in reticulocytes more deeply and more uniformly than does brilliant cresyl blue, which varies from sample to sample in its staining ability.

PROCEDURE. To prepare 10 ml of 6X DNA loading dye, weigh out 25 mg bromophenol blue. Transfer it to a 15-mL screw-capped graduated tube. Add 7.06 ml of 85% Glycerol and 2.94 ml deionized / Milli-Q water.

Bromophenol Blue (BPB) test paper is useful in differentiating between a solution with an acidic pH and one with a more neutral pH. At pH 3.0 and lower, the paper will be a yellow/green color. Above pH 4.6, the paper will remain blue (it is blue when unreacted).

Loading dye is mixed with samples for use in gel electrophoresis. It generally contains a dye to assess how “fast” your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).

1. Dissolve 3.2 g of potassium permanganate in 1000 ml of water.
2. Heat on a water-bath for 1 hour.
3. Allow to stand for 2 days and filter through glass wool.
4. Standardize the solution in the following manner.

For 2N NaOH we have to dissolve 4×2=8grm of NaOH to 100 ml water. Thus by dissolving 8grm of NaOH in 100ml of water we will get 2N NaOH solution.

Dissolve 4.5 g of sodium hydroxide in 100 mL distilled water, allow to cool, and then add saturated barium hydroxide solution drop wise with stirring until a precipitate is formed.

Phenol Red Indicator Solution: Dissolve 0.1 g of phenol red in 2.82 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). After the solution is effected, add sufficient water to produce 100 ml.

Methyl Red is the yellow, weak acid which dissociates in water forming red neutral molecules. … Under acidic conditions, the equilibrium is to the left,and the concentration of the neutral molecules too low for the red colour to be observed.

Thymol blue is another weak acid that is used as an indicator. … The ionization reactions for thymol blue are shown below: At any pH, the color of a thymol blue solution can be predicted using the acid ionization constants. For example, at a pH of 4, the [H+] is 1 x 10-4.

1. – Weigh out 0.5 g of phenolphthalein.
2. – Prepare a 50% ethanol (ethyl alcohol) solution consisting of 50ml ethanol and 50 ml water.
3. – Dissolve the phenolphthalein thoroughly in the 50% ethanol solution.
4. – Use from a bottle fitted with an eye dropper.

Phenolphthalein is naturally colorless but turns pink in alkaline solutions. The compound remains colorless throughout the range of acidic pH levels but begins to turn pink at a pH level of 8.2 and continues to a bright magenta at pH 10 and above.

Phenolphthalein solution 0.375% in methanol.

IndicatorThymol blue (second transition)Low pH coloryellowTransition pH range8.0–9.6High pH colorblue

Bromothymol blue acts as a weak acid in solution. … It is bluish green in neutral solution. The deprotonation of the neutral form results in a highly conjugated structure, accounting for the difference in color. An intermediate of the deprotonation mechanism is responsible for the greenish color in neutral solution.

Pour the diluted bromothymol blue solution into a beaker. Place a straw in the liquid and exhale gently into it. Allow the exhaled air to bubble through the liquid until the solution turns yellow-green. This will take 15 to 30 minutes.

2. Explain that BTB is an indicator that can be used to test for the presence and relative concentration of carbon dioxide.

Why did the color of the Bromthymol Blue (BTB) solution change in certain test tubes? The levels of carbon dioxide changed, therefore the BTB solution changed color to indicate the presence of carbon dioxide.

Why cover one test tube in aluminum foil? The foil blocks light.

Reaction with limewater Carbon dioxide reacts with limewater (a solution of calcium hydroxide, Ca(OH) 2), to form a white precipitate (appears milky) of calcium carbonate, CaCO 3. Adding more carbon dioxide results in the precipitate dissolving to form a colourless solution of calcium hydrogencarbonate.

The Elodea will take up the CO2 and use it to produce sugars during photosynthesis, releasing oxygen as a byproduct. Because of the balanced production and uptake of CO2, the BTB will remain blue.

Methylene Blue (methylene blue) injection is a form of hemoglobin, a substance in blood, used to treat methemoglobinemia. Methylene Blue oral is used to treat methemoglobinemia and urinary tract infections.

1. Take a clean cotton swab and gently scrape the inside of your mouth.
2. Smear the cotton swab on the centre of the microscope slide for 2 to 3 seconds.
3. Add a drop of methylene blue solution and place a coverslip on top. …
4. Remove any excess solution by allowing a paper towel to touch one side of the coverslip.

Dissolve 0.5 g basic fuchsin dye in 20 ml 95% ethanol. Dilute to 100 ml with distilled water. Filter if necessary with Whatman No. 31 filter paper to remove any undissolved dye.