A Gram stain is a test that checks for bacteria at the site of a suspected infection or in certain body fluids, such as blood or urine. … When the stain combines with bacteria in a sample, the bacteria will either stay purple or turn pink or red. If the bacteria stays purple, they are Gram-positive.
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What is the main purpose of gram staining?

Introduction: Gram staining is a method commonly used to determine the chemical make up of the cell wall of bacteria. The cell wall can stain either positive or negative, depending on its chemistry. Knowing the chemical make up makes it easier to manipulate the bacteria for various purposes.

What does a Gram-positive result mean?

If they’re positive, it means bacteria were present. Because of the staining technique used, gram-positive bacteria will appear purple under a microscope and gram-negative bacteria will appear pink. The shape, size, and quantity of bacteria present will also provide information about your infection.

What is the conclusion of gram staining?

CONCLUSION: The Gram stain method is a rapid and cost-effective test that is also highly reproducible and readily available in many laboratories. These features make the Gram stain method a more desirable screening procedure for bacterial vaginosis in a clinic population.

What are four factors that affect the results of the Gram stain?

The many variables that can affect this stain are age of the culture, amount of decolorizer used, the time of decolorization, the type of organism (acid-fast bacteria and spores do not stain well), thickness of the smear, and the general care of the stainer. The most common reasons for false gram reactions?

Is gram-positive bacteria harmful?

Though gram-negative bacteria are harder to destroy, gram-positive bacteria can still cause problems. Many species result in disease and require specific antibiotics.

Why is iodine used in gram staining?

The Gram staining is one of the most crucial staining techniques in microbiology. … The first step in gram staining is the use of crystal violet dye for the slide’s initial staining. The next step, also known as fixing the dye, involves using iodine to form crystal violet- iodine complex to prevent easy removal of dye.

What diseases are caused by Gram positive bacteria?

  • Anthrax. Anthrax may affect the skin, the lungs, or, rarely… …
  • Diphtheria. read more.
  • Enterococcal infections. See also… …
  • Erysipelothricosis. People are infected when they have a puncture wound or scrape while they are handling… …
  • Listeriosis.
What is difference between gram positive and negative?

Gram positive bacteria possess a thick (20–80 nm) cell wall as outer shell of the cell. In contrast Gram negative bacteria have a relatively thin (<10 nm) layer of cell wall, but harbour an additional outer membrane with several pores and appendices.

Is E coli gram positive?

Escherichia Coli is a Common Intestinal Bacteria. E. coli is a Gram negative anaerobic, rod-shaped, coliform bacteria of the genus Escherichia, commonly found in the lower intestine of humans and animals.

What color is gram negative bacteria?

Gram negative organisms are Red. Hint; Keep your P’s together; Purple is Positive. Gram stains are never pink they are red or purple so you don’t destroy the rule; keep your P’s together. In microbiology bacteria have been grouped based on their shape and Gram stain reaction.

What stains are used in Gram staining?

Gram staining involves three processes: staining with a water-soluble dye called crystal violet, decolorization, and counterstaining, usually with safanin.

What Counterstain is used in Gram staining?

Some laboratories use safranin as a counterstain; however, basic fuchsin stains gram-negative organisms more intensely than safranin.

What is the Decolorizer in the Gram stain and why is it important?

The Gram stain is the most important staining procedure in microbiology. … This layer makes up 60-90% of the gram positive cell wall. Decolorizing the cell causes this thick cell wall to dehydrate and shrink, which closes the pores in the cell wall and prevents the stain from exiting the cell.

What can go wrong in Gram staining?

Several factors may affect the results of Gram staining: If the smear is too thick, proper decolorizing will not be possible. If the smear is overheated during heat fixing, the cell walls will rupture. Concentration and freshness of reagents may affect the quality of the stain.

Why 95 Ethanol is used in Gram staining?

Remel Gram Decolorizer (95% Ethyl Alcohol) is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram- positive organisms. The primary stain, crystal violet, is a basic dye which rapidly permeates the cell wall of all bacteria, staining the protoplast purple.