The order of draw is based on CLSI Procedures and Devices for the Collection of Capillary Blood Specimens; Approved Standard – Sixth Edition, September 2008. This standard recommends that EDTA tubes be drawn first to ensure good quality specimen, followed by other additive tubes and finally, serum specimen tubes.
Which blood type has no antibodies? blood type antigens and antibodies chart.

Which blood tube should always be drawn first?

Blood Culture Bottles are ALWAYS drawn prior to other labs to reduce contamination. . Tube MUST be filled 100% – No exceptions! NOTE: All tubes sterile. Standard order of draw: BLOOD CULTURES, royal blue, red, light blue, SST (Gold), green, tan, yellow, pink, pearl, lavender.

What order should blood tubes be drawn?

  1. First – blood culture bottle or tube (yellow or yellow-black top)
  2. Second – coagulation tube (light blue top). …
  3. Third – non-additive tube (red top)
  4. Last draw – additive tubes in this order:
Which vein is the first choice for drawing blood?

The median cubital vein is the first choice for blood draws because it has a decreased proximity to arteries and nerves in the arm. The more lateral cephalic vein is the second choice and the basilic vein in the medial arm is the last choice.

What does a red top tube test for?

Red top – Tube without gel separator, used mainly for serology and chemistry testing. 10mL Red top tubes are used in Blood Bank for antibody screens. c. SST / Gold top – Tube contains a clot activator / gel separator which separates the cells from the serum for a variety of testing.

Does order of draw matter?

In the era of lyophilized anticoagulants, order of draw is no longer important. Contamination of serum samples with K EDTA will occur routinely if order of draw is not followed. … Modern lab equipment can tell the difference between K from the EDTA tube and K from the patient, so order of draw is no longer important.

Why is the order of draw important?

The order of draw is recommended for both glass and plastic venous collections tubes when drawing multiple specimens for medical laboratory testing during a single venipuncture. The purpose is to avoid possible test result error due to additive carryover.

What is the first step in routine blood collection?

The first step to the collection is to positively identify the patient by two forms of identification; ask the patient to state and spell his/her name and give you his/her birth date.

What are the 3 main veins to draw blood?

3.05. The most site for venipuncture is the antecubital fossa located in the anterior elbow at the fold. This area houses three veins: the cephalic, median cubital, and basilic veins (Figure 1).

How do you choose the right vein for venipuncture?

Know Your Veins – A common choice for adult patients is the median cubital vein in the antecubital fossa (think crevice of the elbow). This vein is a good choice for beginners, as it’s close to the surface and tends not to roll when punctured. Some other commonly used veins are the basilic vein and the cephalic vein.

Which vein is the second choice for venipuncture?

The cephalic vein is a safe secondary option if the median cubital vein is not working. This vein can be found along the upper arm to the thumb, but it is commonly drawn from the forearm or antecubital fossa area. Also found in the antecubital fossa, the basilic vein serves as a last resort for blood draws.

What are yellow top tubes used for?

Yellow-top tube (ACD) This tube contains ACD, which is used for the collection of whole blood for special tests. NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen.

What is blood collection procedure?

  • Assemble equipment. …
  • Identify and prepare the patient. …
  • Select the site. …
  • Perform hand hygiene and put on gloves. …
  • Disinfect the entry site. …
  • Take blood. …
  • Fill the laboratory sample tubes. …
  • Draw samples in the correct order.
How do you remember the order of draw?

  1. Boys – Blood Culture.
  2. Love – Light Blue.
  3. Ravishing – Red.
  4. Girls – Gold.
  5. Like – Light Green.
  6. Dieters – Dark green.
  7. Love – Lavender.
  8. Greek – Gray.
What is the correct order of selection of veins in the antecubital fossa?

Venipuncture. The median cubital vein in the antecubital fossa is the most commonly used site due to its accessibility and size, followed by the neighboring cephalic and basilic veins [13,49,51,52].

How do you choose an IV vein?

  1. Start with distal veins and work proximally. Start choosing from the lowest veins first then work upward. …
  2. Use a BP cuff rather than a tourniquet. …
  3. Apply the tourniquet correctly. …
  4. Puncture without a tourniquet.
What is the best vein to start an IV?

I prefer to start IVs in the A.C. region (antecubital fossa). This is the area on the inner fold of the arm. Nurses may also start an IV in the veins on the forearm, back of the arm, or on the hand. Veins in the A.C. region are often larger, so it can be a preferred area when using a larger IV needle.

What vein is on the thumb side?

The cephalic vein runs along the ‘thumb-side’ of the forearm, whereas the basilic vein runs along the ‘pinky-side.

What is green top tube used for?

Lithium Heparin (Green-Top Tube)*: This tube is used for the collection of heparinized plasma or whole blood for special tests. *Note: After the tube has been filled with blood, immediately invert the tube gently several times to prevent coagulation.

What does green top tube test for?

Green top tube with sodium or lithium heparin: used for plasma or whole blood determinations. EDTA tubes: includes Lavender top, Pink top (used for blood bank testing), Tan top (used for lead testing), and Royal Blue top with EDTA (used for trace metal whole blood or plasma determinations).

What is Tiger top tube used for?

Serum Separator Tube (SST) (Tiger Top): Does not contain an anticoagulant but contains a clot activator and serum separator gel. This tube is used for collecting serum.

What are the 4 indications for Venepuncture?

  • to obtain blood for diagnostic purposes;
  • to monitor levels of blood components;
  • to administer therapeutic treatments including medications, nutrition, or chemotherapy;
  • to remove blood due to excess levels of iron or erythrocytes (red blood cells); or.
What is the important step in phlebotomy?

Venipuncture is the process of collecting or “draw- ing” blood from a vein and the most common way to collect blood specimens for laboratory testing. It is the most frequent procedure performed by a phle- botomist and the most important step in this proce- dure is patient identification.

Why must a discard tube should be collected first before an anticoagulated tube when using a butterfly to collect blood?

The discard tube must be used to prime the tubing of the collection set, which will assure maintenance of the proper anticoagulant/blood ratio in the first tube filled. The discard tube should be a non-additive or a coagulation tube, and need not be completely filled.